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Jan 1, 2022 · Add components, except sheep blood and antibiotic solution, to distilled water and bring the volume to 890.0ml and mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 lbs pressure at 121°C. Cool at 45°C-50°C. Aseptically add 100.0ml of sterile sheep blood and 10.0ml of sterile antibiotic solution.

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